A simple, sensitive, precise, accurate and rapid RP-HPLC method has been developed and validated for the simultaneous determination of Diclofenac sodium and Chlorzoxazone in combine dosage form. The RP-HPLC method was performed on sunshell C18 column (100 mm × 4.6 mm i.d., 2.5 μm particle sizes). Mobile phase consisted of a mixture of phosphate buffer (0.05 M KH2PO4, pH adjusted to 3.2 using Orthophosphoric acid): Acetonitrile : TEA (55: 45: 1 v/v/v) at a flow rate of 1.5 ml/min. The detection wavelength was at 240 nm. The proposed method was validated for linearity, accuracy, precision, LOD and LOQ. Retention time of Diclofenac sodium and Chlorzoxazone was 4.94±0.07 & 1.28±0.03, respectively with linearity range of 20-60 µg/mL and 100-300 µg/mL for Diclofenac sodium and Chlorzoxazone respectively. The mean recoveries were 100.19±0.518728 and 99.55±0.536201 for Diclofenac sodium and Chlorzoxazone, respectively. The LOD value were found to be 0.3428 and 4.3289 µg/mL, while LOQ value were found to be 1.0387 and 13.1187 µg /mL for Diclofenac sodium and Chlorzoxazone respectively. This method can now transfer to utilize for routine laboratory analysis and assay of Diclofenac sodium and Chlorzoxazone in their tablet dosage form.
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